OsMADS57 together with OsTB1 coordinates transcription of its target OsWRKY94 and D14 to switch its organogenesis to defense for cold adaptation in rice

Liping Chen, Yuan Zhao, Shujuan Xu, Zeyong Zhang, Yunyuan Xu, Jingyu Zhang, Kang Chong

New Phytologist, 2018, 218(1): 219-231  DOI: 10.1111/nph.14977;      追溯原文......本站官方QQ群：62473826

cold tolerance; D14; gene network; organogenesis; OsMADS57; rice (Oryza sativa); trade-off; WRKY94

1 Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China; 3 National Center for Plant Gene Research, Beijing 100093, China

Plants modify their development to adapt to their environment, protecting themselves from detrimental conditions such as chilling stress by triggering a variety of signaling pathways; however, little is known about how plants coordinate developmental patterns and stress responses at the molecular level.

Here, we demonstrate that interacting transcription factors OsMADS57 and OsTB1 directly target the defense gene OsWRKY94 and the organogenesis gene D14 to trade off the functions controlling/moderating rice tolerance to cold.

Overexpression of OsMADS57 maintains rice tiller growth under chilling stress. OsMADS57 binds directly to the promoter of OsWRKY94, activating its transcription for the cold stress response, while suppressing its activity under normal temperatures. In addition, OsWRKY94 was directly targeted and suppressed by OsTB1 under both normal and chilling temperatures. However, D14 transcription was directly promoted by OsMADS57 for suppressing tillering under the chilling treatment, whereas D14 was repressed for enhancing tillering under normal condition.We demonstrated that OsMADS57 and OsTB1 conversely affect rice chilling tolerance via targeting OsWRKY94.

Our findings highlight a molecular genetic mechanism coordinating organogenesis and chilling tolerance in rice, which supports and extends recent work suggesting that chilling stress environments influence organ differentiation.

OsMADS57與OsTB1一起協(xié)調(diào)其靶基因OsWRKY94和D14的轉(zhuǎn)錄，從而將其器官發(fā)生轉(zhuǎn)化為水稻的冷適應(yīng)防御

植物通過調(diào)節(jié)自身的發(fā)育來適應(yīng)環(huán)境，通過觸發(fā)各種信號通路來保護自己免受諸如低溫脅迫等有害條件的影響；然而，對于植物如何在分子水平上協(xié)調(diào)發(fā)育模式和脅迫反應(yīng)知之甚少。本文我們證實相互作用的轉(zhuǎn)錄因子OsMADS57和OsTB1直接作用于防御基因OsWRKY94和器官發(fā)生基因D14，以權(quán)衡控制/調(diào)節(jié)水稻耐寒性的功能。
　　OsMADS57的過表達維持了低溫脅迫下水稻分蘗的生長。OsMADS57直接與OsWRKY94的啟動子結(jié)合，激活其轉(zhuǎn)錄以應(yīng)對冷應(yīng)激反應(yīng)，而在常溫下抑制其活性。此外，OsWRKY94在正常和低溫條件下直接被OsTB1靶向和抑制。然而，在低溫條件下，OsMADS57直接促進D14的轉(zhuǎn)錄以抑制分蘗，而D14在正常條件下被抑制以促進分蘗條件。我們結(jié)果表明，OsMADS57和OsTB1通過靶向OsWRKY94反過來影響水稻的耐冷性。
　　我們的發(fā)現(xiàn)突出了水稻器官發(fā)生和耐冷性協(xié)調(diào)的分子遺傳機制，支持和擴展了最近關(guān)于低溫脅迫環(huán)境影響器官分化的研究。

►基因列表◄
  TCP轉(zhuǎn)錄因子; 分蘗調(diào)控基因; 莖稈強度 OsTB1; FC1; SCM3; MP3
  多蘗矮稈; 酯酶; 獨腳金內(nèi)酯受體 D14; HTD2; D88; qPPB3
  WRKY轉(zhuǎn)錄因子 OsWRKY94; OsWRKY83
  MADS盒基因 OsMADS57