The brown planthopper (BPH) is the most destructive pest of rice (Oryza sativa) and a substantial threat to rice production, causing losses of billions of dollars annually. Breeding of resistant cultivars is currently hampered by the rapid breakdown of BPH resistance. Thus, there is an urgent need to identify more effective BPH-resistance genes. Here, we report molecular cloning and characterization of Bph3, a locus in rice identified more than 30 years ago that confers resistance to BPH. We show that Bph3 is a cluster of three genes encoding plasma membrane–localized lectin receptor kinases (OsLecRK1-OsLecRK3). Introgression of Bph3 into susceptible rice varieties by transgenic or marker-assisted selection strategies significantly enhanced resistance to both the BPH and the white back planthopper. Our results suggest that these lectin receptor kinase genes function together to confer broad-spectrum and durable insect resistance and provide a resource for molecular breeding of insect-resistant rice cultivars.
褐飛虱（BPH）是最具破壞性的水稻害蟲，對水稻產(chǎn)量造成嚴(yán)重威脅，每年造成數(shù)十億美元的損失。BPH抗性的快速喪失限制了抗性品種的選育。因此，迫切需要鑒定更多有效的BPH抗性基因。本文，我們報道了Bph3的分子克隆和特征，它是30多年前水稻中鑒定的一個抗BPH位點。我們證實Bph3是一個由3個編碼質(zhì)膜凝集素受體激酶（OsLecRK1-OsLecRK3）組成的基因簇。通過轉(zhuǎn)基因技術(shù)或分子標(biāo)記輔助選擇策略將Bph3基因?qū)胍赘兴酒贩N中，能顯著增強水稻對BPH和白背飛虱的抗性。我們的研究結(jié)果表明，這些凝集素受體激酶基因一起發(fā)揮作用，賦予水稻廣譜持久的抗蟲性，并為水稻抗蟲品種的分子選育提供資源。