【目的】篩選聚合抗褐飛虱和抗稻瘟病基因水稻材料，為培育新型抗褐飛虱兼抗稻瘟病的水稻新種質(zhì)提供參考?！痉椒ā坷贸Ｒ?guī)育種、分子標(biāo)記輔助育種和抗病蟲鑒定相結(jié)合的手段，將抗稻褐飛虱基因bph20(t)和bph21(t)以及廣譜高抗稻瘟病基因Pi9聚合到優(yōu)良保持系博ⅢB的遺傳背景中。【結(jié)果】bph20(t)的分子標(biāo)記RM540和BYL7、bph21(t)的分子標(biāo)記RM5348和RM222未在稻褐飛虱抗、感親本間擴(kuò)增出特異條帶，無明顯的多態(tài)性，不能用于該育種群體抗褐飛虱基因bph20(t)和bph21(t)的檢測。Pi9的顯性分子標(biāo)記pB8以及共顯性分子標(biāo)記PB9-1在稻瘟病抗、感親本之間可擴(kuò)增獲得特異條帶,具有多態(tài)性,可用于Pi9基因的檢測。經(jīng)抗蟲鑒定并利用標(biāo)記PB9-1對BC6F2群體的22株材料進(jìn)行PCR擴(kuò)增，含有Pi9基因雜合體的有10株，純合體有6株，不含Pi9基因的有7株。用源自廣西南寧田間感褐飛虱和稻瘟病菌株進(jìn)行多次抗病蟲鑒定，篩選獲得一批抗褐飛虱材料，其中抗性與抗蟲親本BPH54相當(dāng)?shù)牟牧嫌?份，在這6份材料中含有Pi9基因且抗稻瘟病的材料有5份，對稻褐飛虱的抗性與供體親本BPH54水平相當(dāng)。【結(jié)論】通過常規(guī)育種、分子檢測和抗蟲鑒定相結(jié)合的辦法，篩選獲得5份聚合褐飛虱抗性基因（bph20和bph21）和抗稻瘟病基因Pi9的抗或高抗水稻中間材料，為選育新的雙抗保持系和不育系提供種質(zhì)材料。
【Objective】The present study was conducted to screen rice materials pyramiding brown planthopper and rice blast genes for providing reference on breeding new rice germplasms with resistance against brown planthopper (BPH) (Nilaparvata lugens Sta°l) and rice blast caused by the fungus Magnaporthe grisea. 【Method】By using conventional breeding, molecular marker-assisted breeding and BPH resistance bioassay, BPH resistance genes viz., bph20(t) and bph21(t), and the broad-spectrum and highly resistant blast resistance gene Pi9 were pyramided into the genetic background of an elite maintainer line BoIIIB. 【Result】The molecular markers RM540 and BYL7 for bph20(t), as well as RM5348 and RM222 for bph21(t), could not amplify specific bands and showed no obvious polymorphism among the resistant parents and susceptible parents, so they could not be used in molecular marker-assisted selection of the present breeding population. The dominant molecular marker pB8 and codominant molecular marker PB9-1 for Pi9 could amplify specific bands and showed polymorphism among the resistant parents and susceptible parents, and could be used in the molecular marker assisted selection of the present breeding population. The marker PB9-1 was used for PCR amplification of 22 rice plants in BC6F2 population after insect resistant identification, It was found that 10 plants was heterozygote with Pi9 gene, 6 plants was homozygote and 7 plants had no Pi9 gene. By insect resistant identification using rice plants susceptible to brown planthopper and rice blast in field of Nanning, a series of rice plants against to brown planthopper, and 5 accessions had Pi9 gene and resistance to rice blast among 6 accessions with equivalent resistance of parent BPH54. 【Conclusion】By using conventional breeding, molecular detection and insect-disease resistance bioassay, 5 accessions of above resistant rice intermediate materials pyramiding brown planthopper genes and rice blast gene, which could provide germplasm meterials for breeding new double resistant maintainer line and sterile line.