為了進(jìn)一步研究水稻晚粳品種農(nóng)墾58轉(zhuǎn)變?yōu)楣饷艉瞬挥巨r(nóng)墾58S的突變位點(diǎn)，并精細(xì)定位光敏不育基因pms3，我們利用農(nóng)墾58S/1514雜交組合的F1進(jìn)行花藥培養(yǎng)構(gòu)建了一個DH群體，驗(yàn)證了在該群體中光敏不育受pms1、pms3 兩對基因的控制，并根據(jù)分子標(biāo)記分析選擇第12染色體是1514基因型、第7染色體農(nóng)墾58S基因型的DH系DH80與農(nóng)墾58S雜交構(gòu)建一個pms3 光敏不育單基因分離的群體。根據(jù)F3家系的育性分離情況推測出F2 的基因型，結(jié)合分子標(biāo)記分析進(jìn)一步驗(yàn)證了pms3在第12染色體上的位置，并將其定位在RFLP標(biāo)記M36和RZ261之間，與兩標(biāo)記的遺傳距離分別為1.5cM和3.05cM，為啟動pms3區(qū)域的染色體步查打下了基礎(chǔ)。同時還比較分析了RFLP標(biāo)記在DH群體及F2 群體中的分離情況，發(fā)現(xiàn)第12染色體上pms3 區(qū)域在F2群體中正常分離的多個標(biāo)記在DH群體中發(fā)生了嚴(yán)重的偏分離，另外還發(fā)現(xiàn)DH群體第3、7、11染色體上的標(biāo)記也發(fā)生了偏分離。
In order to determine the precise location of pms3 gene which induce Nongken58 (NK58) to PSGMS Nongken58S (NK58S) , a DH population was constructed by culturing F1 anthers of NK58S/ 1514. A line DH80, which was only with PSGMS gene pms1, was selected to cross with NK58S for constructing a segregation population only in pms3 locus. Segregation of RFLP markers was compared between DH and F2 populations. And the results showed that several markers close to pms3 on chromosome 12 normally segregated in F2 populat ion showed obviously distortion segregation in DH population. Meanwhile, some markers on chromosome 3, 7 and 11 in DH population also segregated distortedly. The genotypes for every F3 family were determined by analysis of fertility segregation. And then the location of pms3 and distances between RFLP markers and pms3 were precisely calculated. The gene pms3 is located between M36 and RZ261, but much closer to M36.