【目的】褐飛虱是危害水稻的主要蟲害之一，利用新的抗性基因和通過基因聚合培育抗性品種是目前防治褐飛虱最經(jīng)濟(jì)有效的方法之一?！痉椒ā坑伤幱靡吧狙苌鷣淼乃酒废礏5 對褐飛虱生物型1 和2 具有高度抗性，B5 攜帶的抗性基因Bph14 和Bph15 分別被定位在第3 和第4 染色體上，本研究以B5 為抗褐飛虱供體親本，以優(yōu)良雜交稻親本9311 和1826 為受體親本，通過復(fù)交和回交，分別利用與Bph14 和Bph15 緊密連鎖的SSR 標(biāo)記 MRG2329 和MS5 在分離群體中進(jìn)行分子標(biāo)記輔助選擇，通過苗期分子標(biāo)記檢測和成株期農(nóng)藝性狀選擇，【結(jié)果】最終獲得一系列目標(biāo)基因純合且農(nóng)藝性狀優(yōu)良的穩(wěn)定株系。采用苗期群體鑒定技術(shù)，對其中的38 份重點株系進(jìn)行了抗蟲鑒定，在26 份Bph14 單基因純合株系中，除2 份為中感外，92.31%的材料抗性水平在中抗以上，6 份Bph15 單基因純合株系全為抗或高抗，聚合有Bph14 和Bph15 雙基因的6 份株系抗性都達(dá)到了高抗水平，抗性強(qiáng)于Bph14 或Bph15 單基因純合株系?！窘Y(jié)論】研究表明在育種進(jìn)程中利用與Bph14 和Bph15 緊密連鎖的分子標(biāo)記開展抗褐飛虱分子標(biāo)記輔助育種是一種有效的途徑。
【Objective】 The brown planthopper (BPH) is an important insect pest of rice. One crucial measure for BPH control is to breed resistance cultivars through identification of new BPH resistance genes and pyramiding major resistance genes.【Method】 B5 is a rice line derived from the wild rice Oryza officinalis and is highly resistant to the BPH biotypes 1 and 2. The dominant BPH resistance genes Bph14 and Bph15 have been identified in B5 and mapped on rice chromosome 3 and chromosome 4, respectively. In this study, we used B5 as a BPH resistance donor. The elite restorer lines 9311 and 1826 were used as recipients of the BPH resistance genes in B5. Screened SSR markers MRG2329 and MS5 were used to select BPH resistance genes in the segregating populations. 【Result】 We performed molecular marker-assisted selection (MAS) at the seedling stage and agronomic trait selection at the adult plant stage. We obtained some introgression lines that carried BPH resistance genes and stable agronomic traits. The BPH resistance levels in 38 important lines were determined in the mass seedling screening test. Among 26 Bph14-single introgression lines, 23 lines were resistant or highly resistant, 1 line was moderately resistant, and 2 lines were moderately susceptible. All of the 6 Bph15-single introgression lines showed resistance or high resistance. However, all the 6 Bph14/Bph15 pyramided lines were highly resistant to BPH.【Conclusion】The results indicated that 92.31% Bph14-single introgression lines had moderate resistance to the BPH insect, and the Bph14/Bph15 pyramided lines had higher resistance levels than the single-gene introgression lines. This survey also revealed that the two markers linked with Bph14 and Bph15 were effectively utilized in BPH-resistant breeding programs.