The thermo-sensitive genic male sterility (TGMS) lines play a crucial role in two-line hybrid rice production. For a practical TGMS line, the stability of male sterility is one of the most important technical indicators. In this study, XianS, a spontaneous mutant with stable male sterility from an indica rice cultivar Xianhuangzhan, was classified as a non-pollen type TGMS line. The critical non-pollen sterility point temperature of XianS was determined as 27°C. Genetic analysis demonstrated that the non-pollen sterility in XianS was controlled by a single recessive gene. Using SSR markers and bulked segregant analysis, the TGMS gene in XianS was fine mapped to a 183 kb interval between RMAN81 and RMX21 on chromosome 2. Two markers, 4039-1 and RMX14 completely cosegregated with this gene. Allelism test indicated that the non-pollen phenotype in seven non-pollen type TGMS lines from different sources, XianS, AnnongS-1, Q523S, Q524S, N28S, G421S, and Q527S is caused by the same TGMS gene. Although the location of TGMS gene in XianS is close to the gene OsNAC6, a previously identified candidate gene of tms5 in AnnongS-1, the sequence of OsNAC6 and its promoter region was identical in TGMS line XianS, AnnongS-1, and wild-type Xianhuangzhan. These results suggest that the non-pollen type TGMS trait probably be controlled by the same TGMS gene in different TGMS rice lines, but its real candidate gene still need to be further studied and identified.
溫敏型雄性不育（TGMS）材料對兩系雜交水稻的生產(chǎn)起到很重要作用。對于生產(chǎn)上的溫敏不育材料來說，雄性不育的穩(wěn)定性是重要的技術(shù)指標(biāo)。本研究中，我們從秈稻品種秈黃占的自然突的株系中發(fā)現(xiàn)一無花粉型溫敏型不育的突變體-秈S。秈S的育型轉(zhuǎn)換溫度的臨界點(diǎn)是27℃。遺傳分析表明無花型秈S是受單隱形基因控制的。通過圖位克隆，發(fā)展SSR標(biāo)記及混池分析。我們定位此基因于第二號染色體的RMA81與RMX21兩標(biāo)記的183kb區(qū)間內(nèi)。其中4039-1和RM21兩標(biāo)記與基因共分離。等位分析表明，來源于不同種質(zhì)資源的7種溫敏型雄性育性材料，秈S，安農(nóng)S-1，Q523S，Q52S，N28S，G421S和Q527S屬于同一個溫敏型不育基因，盡管秈S中的TGMS座位靠近OsNAC6-前人報道是tms5突變體的候選基因。對秈S，安農(nóng)S-1，和野生型秈黃占的OsNAC6基因及其啟動子測序，發(fā)現(xiàn)該他們序列無差異。此結(jié)果表明在不同的TGMS的材料中，無花粉型的TGMS性狀很可能是受相同的TGMS基因控制。此基因的真正候選基因有待于進(jìn)一步研究和鑒定。