Inflorescence architecture is one of the most important agronomical traits. Characterization of rice aberrant panicle organization 1 (apo1) mutants revealed that APO1 positively controls spikelet number by suppressing the precocious conversion of inflorescence meristems to spikelet meristems. In addition, APO1 is associated with the regulation of the plastchron, floral organ identity, and floral determinacy. Phenotypic analyses of apo1 and floral homeotic double mutants demonstrate that APO1 positively regulates class-C floral homeotic genes, but not class-B genes. Molecular studies revealed that APO1 encodes an F-box protein, an ortholog of Arabidopsis UNUSUAL FLORAL ORGAN (UFO), which is a positive regulator of class-B genes. Overexpression of APO1 caused an increase in inflorescence branches and spikelets. As the mutant inflorescences and flowers differed considerably between apo1 and ufo, the functions of APO1 and UFO appear to have diverged during evolution.
花序結(jié)構(gòu)是很重要的農(nóng)藝性狀之一。水稻aberrant panicle organization 1 (apo1)突變體的鑒定揭示了APO1通過抑制花序頂端分生組織向小穗分生組織的轉(zhuǎn)變而正調(diào)節(jié)小穗的數(shù)目。APO1參與調(diào)節(jié)葉原基形成間隔期、花器官屬性和花的決定性。對apo1和花器官同源異型轉(zhuǎn)變突變體形成的雙突變體進(jìn)行表型分析表明APO1正調(diào)節(jié)C類花器官同源異型轉(zhuǎn)變基因而不是B類基因。序列分析表明APO1編碼一個F-box蛋白，是擬南芥B類基因正調(diào)節(jié)因子UNUSUAL FLORAL ORGAN (UFO)的直系同源基因。過量表達(dá)APO1引起花序分枝和小穗數(shù)目增加。由于apo1和ufo突變體的花序和花差別很大，因此APO1和UFO的功能在進(jìn)化過程中已發(fā)生分化。
結(jié)果 apo1突變體的表型。 apo1 dl-sup1雙突變體的表型。 apo1-1 spw1-1雙突變體的表型。 APO1的克隆。 APO1的組織表達(dá)模式。 APO1基因的轉(zhuǎn)錄調(diào)節(jié)。
討論 APO1是一個重要的農(nóng)藝基因，影響葉片數(shù)目和穗的大小。 APO1與花器官同源異型轉(zhuǎn)變基因的互作。 APO1的分子功能。 APO1與UFO的功能分化。