Planthoppers are highly destructive pests in crop production worldwide. Brown planthopper (BPH) causes the most serious damage of the rice crop globally among all rice pests. Growing resistant varieties is the most effective and environment-friendly strategy for protecting the crop from BPH. More than 19 BPH-resistance genes have been reported and used to various extents in rice breeding and production. In this study, we cloned Bph14, a gene conferring resistance to BPH at seedling and maturity stages of the rice plant, using a map-base cloning approach. We show that Bph14 encodes a coiled-coil, nucleotide-binding, and leucine-rich repeat (CC-NB-LRR) protein. Sequence comparison indicates that Bph14 carries a unique LRR domain that might function in recognition of the BPH insect invasion and activating the defense response. Bph14 is predominantly expressed in vascular bundles, the site of BPH feeding. Expression of Bph14 activates the salicylic acid signaling pathway and induces callose deposition in phloem cells and trypsin inhibitor production after planthopper infestation, thus reducing the feeding, growth rate, and longevity of the BPH insects. Our work provides insights into the molecular mechanisms of rice defense against insects and facilitates the development of resistant varieties to control this devastating insect.
稻飛虱是世界范圍內(nèi)對(duì)農(nóng)作物生產(chǎn)具有很高破壞性的害蟲(chóng)。在所有的水稻害蟲(chóng)中，褐飛虱是對(duì)水稻造成傷害最嚴(yán)重的的害蟲(chóng)。種植抗褐飛虱的水稻品種是對(duì)付褐飛虱最有效同時(shí)也是對(duì)環(huán)境不造成影響的一種策略。至今已有19個(gè)抗褐飛虱的基因報(bào)道并應(yīng)用與不同地區(qū)的水稻育種和生產(chǎn)中。本文，我們通路圖位克隆的方法克隆了一個(gè)在水稻苗期和成熟期都抗褐飛虱的基因Bph14。Bph14編碼一個(gè)含有卷曲螺旋、核苷酸結(jié)合以及富含亮氨酸重復(fù)序列的蛋白。序列比對(duì)表明Bph14具有一個(gè)獨(dú)特的富含亮氨酸重復(fù)序列，可能起到識(shí)別褐飛虱入侵和激活抗性反應(yīng)的功能。Bph14主要在褐飛虱攝食部位-維管束表達(dá)。Bph14的表達(dá)在褐飛虱侵染之后激活了水楊酸信號(hào)傳導(dǎo)通路，誘導(dǎo)韌皮部細(xì)胞的胼胝質(zhì)沉積以及胰蛋白酶抑制劑的產(chǎn)生，因此降低了褐飛虱的取食、生長(zhǎng)速率和壽命。我們的研究結(jié)果為了解水稻抗蟲(chóng)的分子機(jī)理提供了新的見(jiàn)解，同時(shí)有助于培育抵抗褐飛虱這種破壞性極強(qiáng)的害蟲(chóng)的水稻品種。