Stigma exsertion is an important trait that contributes to the improvement of seed production in hybrid rice. We demonstrate that GS3, one of the genes regulating seed length, also regulates stigma length and participates in stigma exsertion in rice. GS3 mRNA is expressed in the basal part of the young stigma, and a nonsense mutation in the second exon of GS3 causes an increase in cell number, resulting in elongation of the stigma. Manipulation of GS3 should contribute to the improvement of hybrid seed-production efficiency.
柱頭外露率是一個重要的能提高雜交制種中種子生產(chǎn)的性狀。本文中我們證明了GS3是一個在水稻中調(diào)控種子長度和柱頭長度，與柱頭外露率有關(guān)。GS3 的mRNA在幼嫩的柱頭的基部中表達，GS3的第二個外顯子中的一個無義突變使細胞數(shù)目的增加，最終導(dǎo)致柱頭的伸長。GS3的操縱應(yīng)提高雜交制種中種子的生產(chǎn)效率。
結(jié)果：Asominori和AIS22柱頭的比較；互補實驗；GS3在雄蕊中的表達；GS3減少柱頭細胞數(shù)目