Gene pyramiding is considered one of the most effective strategies for achieving durable resistance against blast disease (Magnaporthe oryzae B. Couch) in rice (Oryza sativa L.), although few studies have evaluated the combining effect of the resistance genes. We report the development of pyramided lines with two major blast resistance genes, Pish and Pib, and the evaluation of the combining effect of them. The two genes pyramided lines were selected from the progenies of a cross between one near isogenic line (NIL), which harbours Pish, and another NIL, which harbours Pib, in the genetic background of blast susceptible variety, CO 39. The presence of the resistance genes was confirmed by DNA markers linked to them. To obtain DNA markers for Pish, we genetically mapped the Pish locus. We confirmed the additive effect of Pish and Pib in the pyramided lines by their reaction patterns to blast isolates, suggesting the potential availabilities of the combinations of these genes. In addition, we provide DNA markers linked to Pish for marker aided selection in rice blast resistance breeding.
基因聚合是獲得永久性抗稻瘟病水稻材料的最有效策略之一，盡管很少有研究評估這種基因聚合的效果。本文我們報(bào)道了兩個主效抗稻瘟病基因Pish和Pib聚合系的構(gòu)建，并對它們聚合后的效應(yīng)進(jìn)行評估。這兩個基因的聚合系選自兩個分別攜帶有Pish和Pib的近等基因系雜交后代，這兩個近等基因系的遺傳背景是稻瘟病敏感品種CO39。材料是否攜帶抗性基因得到與它們緊密連鎖的分子標(biāo)記證實(shí)。為了獲得Pish的DNA標(biāo)記，我們對Pish位點(diǎn)進(jìn)行遺傳作圖。我們通過抗性基因與稻瘟病小種的互作模式證實(shí)了Pish和Pib聚合系的附加效應(yīng)，認(rèn)為這兩個基因的聚合系具有潛在利用價(jià)值。此外，我們提供了Pish的緊密連鎖標(biāo)記，有助于水稻抗稻瘟病分子輔助育種。