Pei'ai64S, an indica sterile variety with photoperiod and thermo-sensitive genic male sterile (PTGMS) genes, has been widely exploited for commercial seed production for “two-line” hybrid rice in China. One PTGMS gene from Pei'ai64S, pms1(t), was mapped by a strategy of bulked-extreme and recessive-class approach with simple sequence repeat (SSR) and insert and deletion (In-Del) markers. Using linkage analysis for the F2 mapping population consisting of 320 completely male sterile individuals derived from a cross between Pei'ai64S and 93-11 (indica restorer) lines, the pms1(t) gene was delimited to the region between the RM21242 (0.2 cM) and YF11 (0.2 cM) markers on the short arm of chromosome 7. The interval containing the pms1(t) locus, which was co-segregated with RM6776, is a 101.1 kb region based on the Nipponbare rice genome. Fourteen predicted loci were found in this region by the Institute for Genomic Research (TIGR) Genomic Annotation. Based on the function of the locus LOC_Os07g12130 by bioinformatics analysis, it is predicted to encode a protein containing a Myb-like DNA-binding domain, and may process the transcript with thermosensory response. The reverse transcription-polymerase chain reaction (RT-PCR) results revealed that the mRNA levels of LOC_Os07g12130 were altered in different photoperiod and temperature treatments. Thus, the LOC_Os07g12130 locus is the most likely candidate gene for pms1(t). These results may facilitate not only using the molecular marker assisted selection of PTGMS genes, but also cloning of the pms1(t) gene itself.
培矮64S是一個帶有光溫敏雄性核不育（PTGMS）基因的秈型不育系，在中國兩系雜交水稻制種中被廣泛應用。應用隱性極端群體法，結(jié)合簡單序列重復（SSR）標記和插入缺失（In-Del）標記，定位了一個控制培矮64S育性的PTGMS基因，pms1(t)。利用來源于培矮64S和9311（秈型恢復系）雜交的含有320個完全不育單株的F2定位群體，通過連鎖分析，將pms1(t)定位在水稻第7染色體短臂標記RM21242 (0.2 cM) 和YF11 (0.2 cM)之間。基于日本晴基因組，該區(qū)間為101.1 kb，包含pms1(t)基因位點，目的基因與RM6776共分離?；赥IGR基因組注釋，該區(qū)間內(nèi)包含14個基因?；谏镄畔W對LOC_Os07g12130位點進行功能分析，其編碼一個含有1個Myb-like DNA-binding結(jié)構域的蛋白，并可能參與熱敏感響應的轉(zhuǎn)錄過程。RT-PCR結(jié)果顯示，LOC_Os07g12130在不同光周期和溫度處理下，其轉(zhuǎn)錄水平不同。所以，LOC_Os07g12130被認為是pms1(t)的最有可能的候選基因。本研究將為分子標記輔助選擇利用PTGMS基因，及pms1(t)的克隆奠定基礎。