The Molecular Evolution of the Rice Blast Resistance Gene Pi36

Xin‐Qiong Liu, Ling Wang, Xiang‐Dong Liu, Xue‐Qun Liu, De‐Bin Wang, Chun‐Tai Wang, Fei Lin, Qing‐Hua Pan

International Journal of Plant Sciences, 2010, 171(3): 235-243  DOI: 10.1086/650158;      追溯原文......本站官方QQ群：62473826

Pi36; genomic dynamics; molecular evolutionary; gene conversion

Key Biotechnology Laboratory of the State Ethnic Affairs Commission, College of Life Science, South-Central University for Nationalities, Wuhan 430074, China;
Laboratory of Plant Resistance and Genetics, College of Resources and Environmental Sciences, South China Agricultural University, Guangzhou 510642, China; and
College of Agriculture, South China Agricultural University, Guangzhou 510642, China

The single‐locus rice blast resistance gene Pi36 is of the coiled‐coil NBS‐LRR (nucleotide binding site–leucine‐rich repeat) type. We have investigated its evolution by resequencing a set of haplotypes (six ssp. indica and four ssp. japonica cultivars and three wild rice accessions) both within and outside the gene’s coding region. It was found that the average nucleotide diversity was ～4.5%, characteristic of an intermediately diversified pattern. The K_a/K_s ratio and Tajima’s D test were used to show that the gene’s evolution reflects a mixture of purifying and balancing selection. In all, 186 significant gene conversion events were identified across the full coding region, suggesting that gene conversion has also been an important driver of sequence diversification.

►基因列表◄
  稻瘟病抗性基因 Pi36