分別以保持先B和天B為受體和輪回親本,以攜帶抗白葉枯病基因Xa23的育種材料P59為供體,通過雜交、回交和分子標(biāo)記輔助選擇,結(jié)合農(nóng)藝性狀表現(xiàn)和田間接種抗性鑒定,育成2個(gè)含有Xa23基因且抗白葉枯病的保持系先抗B和天抗B,再分別與先A與天A測(cè)交、成對(duì)回交,育成抗白葉枯病水稻不育系先抗A和天抗A,于2013年9月通過廣西科技廳田間技術(shù)鑒定。這2個(gè)不育系具有敗育徹底、農(nóng)藝性狀優(yōu)良、異交結(jié)實(shí)率高和抗白葉枯病等特點(diǎn),配制的雜交稻組合表現(xiàn)出明顯的白葉枯病抗性和產(chǎn)量?jī)?yōu)勢(shì),具有較好的應(yīng)用前景。
Developed from the hybridization between the breeding material P59 (as the donor of bacterial blight (BB) resistance gene Xa23) and maintainer lines Xian B and Tian B (as the receptor and the recurrent parent in backcrossing), respectively, followed by successive backcrossing with the molecular marker-assisted selection (MAS) and inoculated field identification for BB resistance, Xiankang B and Tiankang B were two rice maintainer lines resistant to BB with the gene of Xa23. They were crossed to Xian A and Tian A respectively and transferred into two new BB-resistant rice CMS lines Xiankang A and Tiankang A with the abovementioned method. Xiankang A and Tiankang A were technically identified by the Department of Science and Technology of Guangxi Zhuang Autonomous Region in September, 2013. They showed complete and stable male sterility, good agronomic traits, high outcrossing rate and BB resistance. The F1 hybrids of these two CMS lines had a good yield heterosis and BB resistance.