Short-chain peptides play important roles in plant development and responses to abiotic and biotic stresses. Here, we characterized a gene of unknown function termed OsDT11, which encodes an 88 amino acid short-chain peptide and belongs to the cysteine-rich peptide family. It was found that the expression of OsDT11 can be activated by polyethylene glycol (PEG) treatment. Compared with wild-type lines, the OsDT11-overexpression lines displayed dramatically enhanced tolerance to drought and had reduced water loss, reduced stomatal density, and an increased the concentration of abscisic acid (ABA). The suppression of OsDT11 expression resulted in an increased sensitivity to drought compared to wild-type expression. Several drought-related genes, including genes encoding abscisic acid (ABA) signaling markers, were also strongly induced in the OsDT11-overexpressing lines. Moreover, the expression of OsDT11 was repressed in ABA-insensitive mutant Osbzip23 and Os2H16 RNAi lines. These results suggest that OsDT11-mediated drought tolerance may be dependent on the ABA signaling pathway.
短肽在植物發(fā)育以及對非生物和生物脅迫的響應(yīng)中發(fā)揮重要作用。本文，我們分析了一個未知功能基因，命名為OsDT11，它編碼一個包含88個氨基酸的短肽，屬于富含半胱氨酸的肽段家族。我們發(fā)現(xiàn)聚乙二醇（PEG）處理會激活OsDT11的表達。與野生型株系相比，OsDT11過表達株系表現(xiàn)出耐旱性顯著增加，水分散失減少，氣孔密度降低，脫落酸（ABA）濃度增加。與野生型株系相比，抑制OsDT11表達會導(dǎo)致對干旱的敏感性增加。在OsDT11過表達株系中，一些干旱相關(guān)基因，包括ABA信號標記編碼基因，也受到強烈誘導(dǎo)。此外，在ABA不敏感突變體Osbzip23和Os2H16-RNAi株系中，OsDT11的表達受到抑制。這些結(jié)果表明，OsDT11介導(dǎo)的耐旱性可能依賴于ABA信號通路。