The genomic expression profile of the super-hybrid rice Liangyoupeijiu female parent Pei’ai 64S in different tissues at different developmental stages under low temperature, drought, and high temperature stresses were detected using an Affymetrix GeneChip Rice Genome Array to screen upregulated and downregulated genes. In this study, we screened the drought-resistant gene OsRCI2-5, after which a constitutive OsRCI2-5 construct was created and transferred into Nipponbare. After polyethylene glycol-6000 and drought treatment, we found that the OsRCI2-5 gene improved the drought resistance of Nipponbare. Gene expression profiling showed that the OsRCI2-5 gene was expressed in the rice leaves, stems, and flower organs. Subcellular localization revealed that the gene was located in the membranes, and hence, we can deduce that a membrane signal peptide was responsible for signal transduction.
低溫、干旱和高溫脅迫下，利用Affymetrix水稻基因組芯片檢測了超級(jí)雜交稻兩優(yōu)培九母本培矮64S在不同生育時(shí)期不同組織中的表達(dá)譜，掃描表達(dá)上調(diào)或下調(diào)的基因。本研究中，我們掃描到一個(gè)干旱抗性基因OsRCI2-5，構(gòu)建組成性OsRCI2-5并轉(zhuǎn)到日本晴中。聚乙二醇6000和干旱處理后，我們發(fā)現(xiàn)OsRCI2-5基因改良了日本晴的耐旱性?；虮磉_(dá)譜表明OsRCI2-5 在水稻葉、莖和花器官中表達(dá)。亞細(xì)胞定位揭示其位于細(xì)胞膜上，因此，我們能推測膜信號(hào)肽對(duì)信號(hào)傳導(dǎo)有應(yīng)答。