The brown planthopper (BPH) is the most serious insect pest of rice in Asia. The indica rice cultivar ADR52 carries two BPH resistance genes, BPH26 (BROWN PLANTHOPPER RESISTANCE 26) and BPH25. Map-based cloning of BPH26 revealed that BPH26 encodes a coiled-coil-nucleotide-binding-site–leucine-rich repeat (CC–NBS–LRR) protein. BPH26 mediated sucking inhibition in the phloem sieve element. BPH26 was identical to BPH2 on the basis of DNA sequence analysis and feeding ability of the BPH2-virulent biotype of BPH. BPH2 was widely incorporated in elite rice cultivars and was well-cultivated in many Asian countries as a favorable gene resource in rice breeding against BPH. However, BPH2 was rendered ineffective by a virulent biotype of BPH in rice fields in Asia. In this study, we suggest that BPH2 can be reused by combining with other BPH resistance genes, such as BPH25, to ensure durable resistance to BPH.
在亞洲，褐飛虱是對(duì)水稻危害性最大的一種害蟲(chóng)。秈稻品種ADR52攜帶有兩個(gè)褐飛虱抗性基因，BPH26和BPH25。對(duì)BPH26進(jìn)行圖文克隆，發(fā)現(xiàn)BPH26編碼一個(gè)CC-NBS-LRR蛋白。BPH26能夠抑制褐飛虱對(duì)韌皮部篩管的吸食。通過(guò)DNA序列分析以及BPH2毒性生物型取食能力鑒定，發(fā)現(xiàn)BPH26和BPH2是同一個(gè)基因。BPH2作為一個(gè)抗褐飛虱育種的優(yōu)異基因資源，被廣泛應(yīng)用于亞洲優(yōu)良品種與推廣品種中。但是在亞洲的水稻生產(chǎn)中，BPH2對(duì)其中一種褐飛虱生物型的致害力抗性較差。我們的結(jié)果表明，BPH2可以聚合其它褐飛虱抗性基因，例如BPH25，以獲得更持久廣泛的褐飛虱抗性。