水稻抗白葉枯病基因Xa23的RFLP標記定位及其STS標記的轉化

樊穎倫, 陳學偉, 王春連, 朱立煌, 章琦, 趙開軍

作物學報, 2006, 32(6): 931-935  DOI: 10.3321/j.issn:0496-3490.2006.06.024;      追溯原文......本站官方QQ群：62473826

Xa23; 水稻白葉枯病; RFLP標記; STS標記

中國農(nóng)業(yè)科學院作物科學研究所;農(nóng)業(yè)部作物遺傳育種重點實驗室;農(nóng)作物基因資源與基因改良國家重大科學工程;中國科學院遺傳與發(fā)育生物學研究所;國際水稻研究所中國辦事處

用水稻抗白葉枯病基因Xa23的近等基因系CBB23與其感病輪回親本金剛30(JG30)雜交，構建了包含2562個單株的F2作圖群體.用水稻白葉枯病廣致病菌系P6進行抗性鑒定表明，F(xiàn)2植株抗感分離比嚴格符合3:1.根據(jù)日本水稻基因組計劃RGP水稻高密度圖譜上的RFLP探針對F2群體中的145個感病單株進行RFLP檢測和連鎖分析，獲得了6個與Xa23緊密連鎖的RFLP分子標記.其中RFLP標記C1003A靠著絲粒一側，與Xa23的遺傳圖距為0.4 cM，為Xa23的圖位克隆奠定了重要基礎.并將標記C1003A成功地轉化為STS標記，為分子標記輔助選擇育種(MAS)提供了方便有效的分子標記.

Mapping the Rice Bacterial Blight Resistance Gene Xa23 with RFLP Markers and Converting RFLP to STS Marker

FAN Ying-Lun, CHEN Xue-Wei, WANG Chun-Lian, ZHU Li-Huang, ZHANG Qi, ZHAO Kai-Jun

Acta Agronomica Sinica, 2006, 32(6): 931-935

Xa23; Rice bacterial blight; RFLP markers; STS marker

A F2 population derived from cross of the Xa23 near isogenic lines, JG30 and CBB23, was used for fine mapping of the ricebacterial blight resistance gene Xa23 1 Inoculation of the F2 plants with a Xanthomonas oryzae strain P6 showed a 3: 1 ratio for thesegregation of resistant and susceptible as expected1 One hundred and forty-five susceptible plants were selected from the F2 population formapping the gene Xa23 and fifteen restriction fragment length polymorphism ( RFLP) markers anchored on the rice high-density map1 ARFLP marker C1003A was found to be linked to the gene Xa23 by 014 cM1 This result constitutes an important basis for map-based cloningof the gene Xa231 The RFLP marker C1003A was transformed into sequence-tagged-site ( STS) marker, which can be applied for molecularmarke-r assisted selection (MAS) of Xa23 in rice breeding programs.

►基因列表◄
  白葉枯病抗性基因 Xa23