以攜帶抗白葉枯病基因Xa23的抗病品系‘中野5112'為抗源，以優(yōu)良水稻品種‘中稻88'和‘中粳Q-196'為受體材料，采用雜交和回交在分離群體中利用與Xa23緊密連鎖的EST標記C189進行分子標記輔助選擇，通過分子標記檢測和成株期農藝性狀選擇，獲得70份目標基因純合且農藝性狀穩(wěn)定的株系.使用鑒別菌系P6，采用人工剪葉接種法，在孕穗期對44份重點株系進行抗性鑒定，所有株系在孕穗期都表現(xiàn)抗病.結果表明利用與Xa23基因緊密連鎖的分子標記C189開展抗白葉枯病分子標記輔助育種是一簡便、有效的途徑.
Abstract: The bacterial leaf blight ( BB) resistant gene Xa23 from Zhongye 5112 was introduced into a restorer lineZhongdao 88 and Zhongjing Q-196，which is susceptible to BB by crossing and marker-assisted selection． The Xa23-linked EST marker C189 was used to select Xa23 in the off-spring． Marker-assisted selection ( MAS) for Xa23 wasperformed at booting stages，and followed by conventional selection for agronomic traits at the ripening stage． Morethan 70 introgression lines that harbored homozygous Xa23 and showed stable agronomic traits were obtained． Fortyfourof the introgression lines were subject to BB disease inoculation at the booting stages， and results showed that allthe tested lines were resistant to the most destructive Xoo strain PXO 99 ( P6) ． These results clearly suggested thatthe Xa23-linked marker C189 could be conveniently and effectively used in the BB-resistance breeding programs．