以抗白葉枯病的華恢20（攜帶Xa7和Xa21基因）和抗螟蟲的T1c-19（攜帶cry1C*基因）為供體，分別與先恢207進行雜交、回交和復交，運用分子標記輔助選擇技術，將白葉枯病抗性基因Xa7、Xa21和螟蟲抗性基因cry1C*聚合到水稻恢復系先恢207中，育成了2個攜帶Xa7+Xa21+cry1C*基因的改良恢復系株系，編號為CY10038-1和CY10039-1。再與金23A配制了2個組合金23A/CY10038-1和金23A/CY10039-1。通過對改良品系及其雜交組合白葉枯病和螟蟲抗性鑒定、CRY1C*蛋白表達量測定以及對產(chǎn)量、稻米品質、生育期和主要農(nóng)藝性狀的分析，結果表明，2個改良系及其雜交組合金23A/CY10038-1和金23A/CY10039-1均抗ZHE173、GD1358、PXO61、PXO99、FuJ、YN24和HeN11等7個白葉枯病菌株；盡管2個雜交組合CRY1C*蛋白表達量低于其改良系，但在全生育期不防治的栽培條件下不受螟蟲危害；2個雜交組合單株產(chǎn)量比金優(yōu)207均明顯提高，而稻米品質和主要農(nóng)藝性狀與金優(yōu)207相似，具有良好的應用前景。
Xianhui 207, the male parent of widely-used hybrid rice Jinyou 207 (Jin 23A/Xianhui 207), was used as the recipient parent to cross with two resistance varieties, Huahui 20 carrying Xa7 and Xa21 genes and T1c-19 carrying cry1C* gene, as donor parents, to improve the bacterial blight (BB) and borer resistance of Xianhui 207. Two improved lines, designated as CY10038-1 and CY10039-1, carrying the genes of Xa7, Xa21 and cry1C*, were developed by hybridizing, backcrossing and multi-crossing with the method of molecular marker-assisted selection. Crossed with Jin 23A, two hybrids Jin 23A/CY10038-1 and Jin 23A/CY10039-1 were made. The resistances of bacterial blight, leaf folder and stem borer, the expression of CRY1C* protein and main agronomic traits including yield and grain quality were studied for the new improved lines and their derived hybrids. The results showed that two improved lines and their hybrids were resistant to seven Xoo strains, i.e., ZHE173, GD1358, PXO61, PXO99, FuJ, YN24 and HeN11. Though the CRY1C* expression in two hybrids was lower than that in the improved lines, the hybrids were highly resistant to leaf folder and stem borer in field conditions. The testing results of main agronomic traits and grain quality showed that two hybrids were similar to Jinyou 207 except for their higher yield than that of Jinyou 207. Thus, these new improved lines would have a good prospect in future hybrid rice breeding.