The brown planthopper (BPH) is one of the most serious insects pests of rice, and the host resistance has been recognized as one of the most economic and effective measures for BPH management. In this study, we conducted a molecular-based genetic analysis of Bph9 in Kaharamana, a Sri Lanka rice variety resistant to BPH insects of East and Southeast Asia. An F2 segregating population composed of 180 plants was constructed from the cross between Kaharamana and 02428, and each F2 plant was self-crossed to obtain F2:3 family. The bulked seedling test method was used to evaluate the resistance of F2:3 families, and the genotype of each F2 plant was inferred from the phenotype of corresponding F2:3 family. Linkage analysis indicated that the resistant gene Bph9 in Kaharamana was located between SSR markers RM463 and RM5341 on chromosome 12 with linkage distances of 6.8 cM and 9.7 cM, respectively. The time- and money-saving SSR markers would be helpful in the application of Bph9 in breeding program via marker-assisted selection.
褐飛虱是危害水稻生產(chǎn)最重要的害蟲之一，利用寄主抗性被認(rèn)為是防治褐飛虱最經(jīng)濟(jì)而有效的方法。斯里蘭卡水稻品種Kaharamana對(duì)東亞和東南亞的褐飛虱種群均表現(xiàn)抗蟲性，利用分子遺傳學(xué)的方法對(duì)其攜帶的Bph9基因進(jìn)行了SSR定位。所用的遺傳群體為來源于Kaharamana和02428的含有180個(gè)單株的F2分離群體，每個(gè)F2單株套袋自交獲得F2:3家系。利用苗期集團(tuán)鑒定對(duì)F2:3家系進(jìn)行抗褐飛虱鑒定，以推測(cè)相應(yīng)F2單株的基因型。連鎖分析表明，Bph9位于第12染色體上的兩個(gè)SSR標(biāo)記RM463和RM5341之間，分別與之相距6.8cM和9.7cM。該標(biāo)記有助于將Bph9用于分子標(biāo)記輔助選擇育種研究。