【目的】為采用分子標記輔助選擇利用野生稻抗性基因提供科學依據(jù)，并為選育超級雜交水稻提供目標親本和選育新的恢復系提供親本。【方法】以白葉枯病抗性水稻中野5112為供體親本、廣親和恢復系桂649為受體親本進行有性雜交，將在分離群體中篩選的35份優(yōu)良目標品系進行田間接菌鑒定，并用與水稻抗白葉枯病基因Xa23緊密連鎖的SSR標記引物RM206對中野5112、恢復系桂649及其35份優(yōu)良雜交后代F4材料進行分子檢測?！窘Y果】從桂649×中野5112雜交后代的35個F4品系中，篩選出13個同時具有白葉枯病抗性和廣親和性潛力及其他優(yōu)良農(nóng)藝性狀的品系，其中屬偏粳型11個，屬偏秈型的僅有兩個；供體材料中野5112及其13份雜交后代材料攜有Xa23的抗性等位點，受體材料桂649及其他22份雜交后代材料未攜帶Xa23的抗性等位點。田間接菌鑒定結果表明，在37個檢測材料中，除中野5112外，對白葉枯病表現(xiàn)中抗以上的有18個品系，進一步證明這部分材料已攜帶有Xa23基因。將13份攜有Xa23抗性等位點的材料與秈、粳稻品種及不育系配組，根據(jù)其F1代的育性表現(xiàn)，證明其中12個品系具有廣親和性。此外，與對照汕優(yōu)63相比，初步鑒定出適合廣西種植的具有超高產(chǎn)潛力的雜交稻組合3個，即龍?zhí)馗×100-1-4、龍?zhí)馗×08262-2-1和龍?zhí)馗×08278-1-1?！窘Y論】采用SSR標記引物與PCR檢測方法進行Xa23基因分子標記檢測是可行的，這為采用分子標記輔助選擇利用野生稻抗性基因提供科學依據(jù)。篩選出的13份優(yōu)良品系可用作選育超級雜交水稻的目標親本或選育新恢復系的親本。
【Objective】The present experiment was conducted to provide scientific experimental data for utilization of disease resistant genes in wild rice (Oryzae rufipogon) using marker-assisted selection (MAS) and also to provide desirable target parental lines for breeding super hybrid rice and source parental lines for selecting new restorer lines. 【Method】The bacterial leaf blight (BLB) resistance gene donor Zhongye 5112 was crossed with the acceptor wide compatible restorer line Gui 649 for selection of their 35 F4 offsprings which were detected by using PCR-based molecular marker RM206, closely linked to BLB resistance gene Xa23. The field inoculation tests of BLB were also performed at the same time. 【Results】Amongst 35 F4 hybrid offsprings, 13 (11 japonica and 2 indica) showed potential of BLB-resistance, wide compatibility and better comprehensive agronomic traits. The allele of Xa23 appeared in the donor Zhongye 5112 and its 13 F4 offsprings, while it was absent in acceptor Gui 649 and other 22 offsprings. The field artificial inoculation results indicated that amongst 37 rice lines, 18 lines showed moderate BLB-resistance except for Zhongye 5112, which proved that these lines contained Xa23 gene. Furthermore, the 13 lines with gene Xa23 were crossed to indica and japonica tester varieties and male sterile lines for evaluation of their wide compatibility and hybrid heterosis. The results showed that 12 lines with Xa23 gene had wide compatibility based on the seed setting rate of their F1 hybrids. As compared to control variety Shanyou 63, 3 hybrid combinations with super high yield and desirable hybrid heterosis, viz., Longtefu A×100-1-4, Longtefu A×08262-2-1 and Longtefu A×08278-1-1 were identified for planting in rice producing areas of Guangxi. 【Conclusion】The results of marker assisted detection of BLB resistance in different rice lines were coincided with those of field artificial inoculation, which indicated that the gene Xa23 could be detected with PCR-based molecular marker RM206 used in this study. The experimental data could be used for utilization of disease resistance genes of wild rice (Oryzae rufipogon) through marker assisted selection (MAS). Thirteen elite lines selected in this study can be used as desirable target parental lines in breeding super hybrid rice and as source parental lines in selection of new restorer lines.