攜有抗白葉枯病新基因Xa23水稻近等基因系的構建及應用(英文)

章琦, 王春連, 趙開軍, 楊文才, 喬楓, 周永力, 江祺祥, 劉古春

中國水稻科學, 2002, 16(3): 206-210     追溯原文......本站官方QQ群：62473826

近等基因系; 育種; 抗性; 基因; 白葉枯病; 水稻

中國農(nóng)業(yè)科學院作物育種栽培研究所;農(nóng)業(yè)部作物遺傳育種重點實驗室;中國農(nóng)?

1993～ 1998年，構建了攜有抗稻白葉枯病新基因 Xa2 3的近等基因系，命名為 CBB2 3。以全生育期高度感病并具有改良株型的秈稻品種 JG30為輪回親本，與攜有 Xa2 3的抗性供體 H4雜交，JG30 / H4的 F1 通過回交、自交直至 B5F4，各世代均用與目標基因 Xa2 3相對應的專化小種菌系 P6人工接種鑒定，同步進行株型和農(nóng)藝性狀選擇，直至抗性穩(wěn)定和農(nóng)藝性狀類似其輪回親本。比較了 CBB2 3(攜 Xa2 3)和 IRBB2 1(攜 Xa2 1)對 2 0個菌系包括 10個菲律賓小種、3個日本小種和7個中國病原型代表菌系的抗譜，Xa2 3抗所有 2 0個菌系；Xa2 1抗 19個菌系，對菲律賓 10號小種則高度感病。用近等基因系 CBB2 3構建了 JG30 / CBB2 3組合的 F2 分離群體，通過 SSR標記篩選，初步將 Xa2 3定位于水稻第 11染色體。進一步篩選出 3個與 Xa2 3更緊密連鎖的 AFL P標記。其中的 APKj2 3與 Xa2 3之間的圖距約為 1.0 c M。并報道了在抗病育種中已有效的應用了攜有 Xa2 3的近等基因系 CBB2 ...

Development of Near-Isogenic Line CBB23 with a New Resistance Gene to Bacterial Blight in Rice and Its Application

ZHANG Qi, WANG Chun lian, ZHAO Kai jun, YANG Wen cai, QIAO Feng, ZHOU Yong li, JIANG Qi xiang, LIU Gu chun

Chinese Journal of Rice Sciences, 2002, 16(3): 206-210

near isogenic line; breeding; resistance; gene; bacterial blight; rice

The near isogenic line (NIL) with a new gene Xa23 for resistance to bacterial blight (BB) was developed and designated as CBB23 in 1993-1998. A susceptible indica variety JG30 with improved plant type was used as a recurrent parent. The progenies derived from the cross JG30/H 4 harboring the gene Xa23 were developed by back cross until BC 5, and selfing for 4 times. Evaluation of the resistance gene Xa23 with specific strain P6 and selection of plant type successively were carried out till the line show...

►基因列表◄
  白葉枯病抗性基因 Xa23