為了研究稻瘟病抗性基因Pi36的進(jìn)化機(jī)理，以孟加拉國(guó)普通野生稻W(wǎng)39、馬來(lái)西亞普通野生稻W(wǎng)40、印度野生稻L56為材料，對(duì)Pi36等位基因進(jìn)行了克隆和序列分析。測(cè)序結(jié)果與已知的Pi36和測(cè)序品種日本晴以及93-11的基因序列進(jìn)行比較。結(jié)果表明，編碼區(qū)6個(gè)品種間的同源性為83%～99%，CC-NBS區(qū)域的同源性平均為91%；LRR區(qū)域?yàn)?5%。并且還發(fā)現(xiàn)在野生稻W(wǎng)39和W40基因的第二個(gè)外顯子區(qū)域有一個(gè)轉(zhuǎn)座子插入。進(jìn)化分析表明，6個(gè)品種從整體上分為3類(lèi)，L56、日本晴和Pi36聚為一類(lèi)，說(shuō)明這3個(gè)品種的親緣關(guān)系較近；W39和W40聚為另一類(lèi)；93-11的基因聚為單獨(dú)的一類(lèi)，處于獨(dú)立進(jìn)化的模式。
To study evolution mechanism of the blast resistance gene, Pi36 and its allele were cloned and sequenced from common wild rice W39, W40 and L56, which were originated from Bangladesh, Malaysia and India.The gene sequences were analyzed including known Pi36 and reference sequenced cultivars Nipponbare and 93-11.The results showed that the nucleotide identity of ORF was 83%～99% among the six cultivars, CC-NBS with 91% and LRR with 95%.Interestingly, a transposon element was inserted in the second exon of W39 ...