通過優(yōu)化PCR擴增體系，利用長片段PCR技術(shù)擴增全長稻瘟病抗性基因Pi36。結(jié)果表明，利用擴增長片段的LA Taq酶，結(jié)合使用GC buffer I，以及熱啟動PCR技術(shù)和兩步法擴增，在退火溫度為62℃和62.8℃時，得到了擴增效率較高，特異性高的16.5kb目標帶。
To clone the full-length blast resistance gene Pi36,the gene was amplified by long-range PCR technique,in which the PCR amplification system was analyzed.The results showed that the target fragment were amplified at 62 ℃and 62.8 ℃ of the annealing temperature,in combination with LA Taq enzyme and GC buffer I.Moreover,hot start and two-step PCR were required for successful amplification.The results would aid the dissection the function of the resistance gene Pi36.