| ·常規(guī)信息 最近更新：2024年5月11日 10:11:00 | |||||||||||
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| 基因(座)名稱 | 生長素受體 auxin receptor; auxin transport inhibitor response 1 | ||||||||||
| 基因符號 | OsTIR1 | ||||||||||
| 所在染色體 | 5 （已克隆） | ||||||||||
OsTIR1(LOC_Os05g05800, Li et al. 2016; Os05g05800, Lu et al. 2018)... 【生物學(xué)功能】OsTIR1是OsmiR393的靶標(biāo)（Xia et al. 2012; Bian et al. 2012），在調(diào)節(jié)旗葉傾角和以及主根和不定根生長中起作用（Bian et al. 2012）。 OsmiR393通過對腋芽中生長素信號傳導(dǎo)的調(diào)控，參與氮促進的水稻分蘗。N誘導(dǎo)OsmiR393積累，降低靶標(biāo)基因OsTIR1和OsAFB2的表達，這會減輕葉腋中生長素的敏感性并使OsIAA6穩(wěn)定，進而促進水稻分蘗（Li et al. 2016）。 TGW3/OsGSK5與OsIAA10互作并將其磷酸化。OsIAA10的磷酸化，增加了其與OsTIR1間的互作和自身蛋白降解，同時也降低了其與OsARF4間的互作。遺傳和分子證據(jù)表明，OsTIR1-OsIAA10-OsARF4模塊是水稻籽粒大小控制的關(guān)鍵。TGW3還介導(dǎo)了水稻植株對油菜素內(nèi)酯的反應(yīng)，而且該效應(yīng)能夠通過上述信號通路進行傳遞（Ma et al. 2023）。 水稻籽粒灌漿過程中，OsTIR1介導(dǎo)生長素信號轉(zhuǎn)導(dǎo)，正調(diào)控胚乳發(fā)育。相比野生型，突變體tir1的株高、每穗粒數(shù)、粒長、粒寬和粒重降低，胚乳中總淀粉和直鏈淀粉含量下降，淀粉合成基因表達上調(diào)，蛋白含量升高，單株產(chǎn)量下降，根系生長對外源生長素2,4-D的敏感性下降，而過表達OsTIR1的表現(xiàn)相反。生長素應(yīng)答因子基因中，僅OsARF25轉(zhuǎn)錄水平在tir1中被抑制，在OsTIR1過表達系中增強，其最高轉(zhuǎn)錄水平出現(xiàn)在受精后10天，與OsTIR1表達一致。OsARF25在體內(nèi)外可以結(jié)合糖轉(zhuǎn)運蛋白基因OsSWEET11的啟動子。突變體arf25和arf25/sweet11表現(xiàn)出淀粉含量和種子大小降低，類似于tir1突變體（Wu et al. 2024）。 【相關(guān)登錄號】
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| ·ONTOLOGY及相關(guān)基因 | |
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| 表型特征 | 株高(TO:0000207), 分蘗數(shù)(TO:0000346), 葉角(TO:0000206), 單株穗數(shù)(TO:0000152), 每穗粒數(shù)(TO:0002759), 粒寬(TO:0000402), 脫殼谷粒長度(TO:0000734), 總淀粉含量(TO:0000696), 胚乳貯藏蛋白含量(TO:0002653), 種子重量(TO:0000181), 千粒重(TO:0000592), 生長素敏感性(TO:0000163), 單株產(chǎn)量(TO:0000449), 種子長度(TO:0000146), 種子寬度(TO:0000149), 籽粒灌漿速率(TO:1002661) |
| 分子功能 | 生長素受體活性(GO:0038198) |
| 生物進程 | 生長素信號轉(zhuǎn)導(dǎo)(GO:0009734), 碳水化合物轉(zhuǎn)運(GO:0008643), 胚乳發(fā)育調(diào)控(GO:2000014), 淀粉生物合成調(diào)控(GO:0010581), 分蘗形成調(diào)控(GO:2000032), 種子發(fā)育調(diào)控(GO:0080050), 根發(fā)育調(diào)控(GO:2000280) |
| ·參考文獻 |
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| 1. Daxia Wu;Yanan Cao;Daojian Wang;Guoxinan Zong;Kunxu Han;Wei Zhang;Yanhua Qi;Guohua Xu;Yali Zhang Auxin receptor OsTIR1 mediates auxin signaling during seed filling in rice Plant Physiology, 2024, 194(4): 2434-2448 |
| 2. Ming Ma;Shao-Yan Shen;Chen Bai;Wei-Qing Wang;Xiao-Hui Feng;Jie-Zheng Ying;Xian-Jun Song Control of grain size in rice by TGW3 phosphorylation of OsIAA10 through potentiation of OsIAA10-OsARF4-mediated auxin signaling Cell Reports, 2023, 42(3): 112187 |
| 3. Fu Guo;Yizi Huang;Peipei Qi;Guiwei Lian;Xingming Hu;Ning Han;Junhui Wang;Muyuan Zhu;Qian Qian;Hongwu Bian Functional analysis of auxin receptor OsTIR1/OsAFB family members in rice grain yield, tillering, plant height, root system, germination, and auxinic herbicide resistance New Phytologist, 2021, 229(5): 2676-2692 |
| 4. Yuzhu Lu;Zhen Feng;Xuanyu Liu;Liying Bian;Hong Xie;Changlun Zhang;Kirankumar S. Mysore;Jiansheng Liang MiR393 and miR390 synergistically regulate lateral root growth in rice under different conditions BMC Plant Biology, 2018, 18: 261 |
| 5. Xiang Li;Kuaifei Xia;Zhen Liang;Kunling Chen;Caixia Gao;Mingyong Zhang MicroRNA393 is involved in nitrogen-promoted rice tillering through regulation of auxin signal transduction in axillary buds Scientific Reports, 2016, 6: 32158 |
| 6. Kuaifei Xia;Ren Wang;Xiaojin Ou;Zhongming Fang;Changen Tian;Jun Duan;Yaqin Wang;Mingyong Zhang OsTIR1 and OsAFB2 Downregulation via OsmiR393 Overexpression Leads to More Tillers, Early Flowering and Less Tolerance to Salt and Drought in Rice PLoS ONE, 2012, 7(1): e30039 |
| 7. Hongwu Bian;Yakun Xie;Fu Guo;Ning Han;Shengyun Ma;Zhanghui Zeng;Junhui Wang;Yinong Yang;Muyuan Zhu Distinctive expression patterns and roles of the miRNA393/TIR1 homolog module in regulating flag leaf inclination and primary and crown root growth in rice (Oryza sativa) New Phytologist, 2012, 196(1): 149-161 |
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