| ·常規(guī)信息 最近更新：2021年5月29日 19:43:00 | |||||||||||
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| 基因(座)名稱 | 蔗糖轉(zhuǎn)運蛋白; 白葉枯病抗性基因; 白葉枯病易感基因 sugar transporter; Bacterial Blight resistance 41; bacterial blight susceptibility gene | ||||||||||
| 基因符號 | Os-11N3; xa41(t); OsSWEET14 | ||||||||||
| 所在染色體 | 11 （已克�。� | ||||||||||
Os-11N3(Os11g31190, Hutin et al. 2015; LOC_Os11g31190, Blanvillain-Baufumet al. 2017)... 【突變體表型】Os-11N3插入突變或RNAi沉默后，對那些依賴病原菌效應子AvrXa7或PthXo3的致病小種的特異感病性喪失。誘導感病基因Os-11N3表達，能破壞水稻白葉枯隱性抗性基因xa13的作用（Antony et al. 2010）。 利用CRISPR/Cas9敲除OsSWEET14，與野生型中花11相比，轉(zhuǎn)基因植株株高增加8%，對白葉枯病菌抗性增強，但產(chǎn)量性狀沒有變化（Zeng et al. 2020）。 接種紋枯病菌AG1-IA后，SWEET14超表達株系對紋枯病菌敏感度降低，表明SWEET14可能會降低細胞間系中的糖含量，從而抑制立枯絲核菌生長，提高水稻紋枯病抗性，然而超表達植株出現(xiàn)異常矮化，千粒重和每穗粒數(shù)降低；SWEET14敲除突變體的生長和產(chǎn)量是正常的（Kim et al. 2021）。 【時空表達譜】qRT-PCR分析表明OsSWEET14在莖稈中表達水平最高，其次是葉鞘和葉片（Zeng et al. 2020）。 【生物學功能】糖轉(zhuǎn)運蛋白SWEET14正調(diào)控水稻對紋枯病的抗性，然而SWEET14過表達對糖的非特異性運輸顯著降低了產(chǎn)量。DOF11調(diào)控SWEET14轉(zhuǎn)錄，DOF11過表達增加了對紋枯病抗性，但也降低了產(chǎn)量，而組織特異性表達VP16-DOF11融合蛋白，則既可以增加產(chǎn)量，也能提高對紋枯病的抗性（Kim et al. 2021）。 效應子AvrXa7可以與Os-11N3啟動子特異結合，驅(qū)動Os-11N3表達，這支持了之前預測的TAL效應子特異結合模型（Antony et al. 2010）。 OsSWEET14編碼一個蔗糖轉(zhuǎn)運蛋白。OsSWEET14等位基因xa41(t)，抗白葉枯�。℉utin et al. 2015）。 非洲白葉枯病菌效應子Tal5能誘導OsSWEET14表達，有利于病菌毒性（Streubel et al. 2013）。 【相關登錄號】
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| ·ONTOLOGY及相關基因 | |
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| 表型特征 | 白葉枯病抗性(TO:0000175), 株高(TO:0000207), 紋枯病抗性(TO:0000255), 每穗粒數(shù)(TO:0002759), 蔗糖含量(TO:0000328), 千粒重(TO:0000592), 穗大小(TO:0006032), 單株產(chǎn)量(TO:0000449) |
| 分子功能 | 糖跨膜轉(zhuǎn)運蛋白活性(GO:0051119) |
| 生物進程 | 穗發(fā)育(GO:0010229), 節(jié)間發(fā)育模式建成(GO:0080006), 抗真菌反應調(diào)控(GO:1900150), 種子發(fā)育調(diào)控(GO:0080050), 抗細菌反應調(diào)控(GO:1900424), 蔗糖跨膜轉(zhuǎn)運(GO:1904982) |
| ·參考文獻 |
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| 1. Dangping Luo;Jose C. Huguet-Tapia;R. Taylor Raborn;Frank F. White;Volker P. Brendel;Bing Yang The Xa7 resistance gene guards the rice susceptibility gene SWEET14 against exploitation by the bacterial blight pathogen Plant Communications, 2021, 2(3): 100164 |
| 2. Pyol Kim;Cai Yun Xue;Hyon Dok Song;Yue Gao;Lu Feng;Yuhua Li;Yuan Hu Xuan Tissue-specific activation of DOF11 promotes rice resistance to sheath blight disease and increases grain weight via activation of SWEET14 Plant Biotechnology Journal, 2021, 19(3): 409-411 |
| 3. Xuan Zeng;Yufen Luo;Nga Thi Quynh Vu;Shujuan Shen;Kuaifei Xia;Mingyong Zhang CRISPR/Cas9-mediated mutation of OsSWEET14 in rice cv. Zhonghua11 confers resistance to Xanthomonas oryzae pv. oryzae without yield penalty BMC Plant Biology, 2020, 20: 313 |
| 4. Abha Zaka;Genelou Grande;Thea Coronejo;Ian Lorenzo Quibod;Chun-Wei Chen;Su-Jein Chang;Boris Szurek;Muhammad Arif;Casiana Vera Cruz;Ricardo Oliva Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae PLoS ONE, 2018, 13(9): e0203711 |
| 5. Servane Blanvillain‐Baufumé;Maik Reschke;Montserrat Solé;Florence Auguy;Hinda Doucoure;Boris Szurek;Donaldo Meynard;Murielle Portefaix;Sébastien Cunnac;Emmanuel Guiderdoni;Jens Boch;Ralf Koebnik Targeted promoter editing for rice resistance to Xanthomonas oryzae pv. oryzae reveals differential activities for SWEET14‐inducing TAL effectors Plant Biotechnology Journal, 2017, 15(3): 306-317 |
| 6. Mathilde Hutin;Fran?ois Sabot;Alain Ghesquière;Ralf Koebnik;Boris Szurek A knowledge-based molecular screen uncovers a broad-spectrum OsSWEET14 resistance allele to bacterial blight from wild rice The Plant Journal, 2015, 84(4): 694-703 |
| 7. Jana Streubel;Céline Pesce;Mathilde Hutin;Ralf Koebnik;Jens Boch andBoris Szurek Five phylogenetically close rice SWEET genes confer TAL effector-mediated susceptibility to Xanthomonas oryzae pv. oryzae New Phytologist, 2013, 200(3): 808-819 |
| 8. Ting Li;Bo Liu;Martin H Spalding;Donald P Weeks;Bing Yang High-efficiency TALEN-based gene editing produces disease-resistant rice Nature Biotechnology, 2012, 30(5): 390-392 |
| 9. Ginny Antony;Junhui Zhou;Sheng Huang;Ting Lib;Bo Liu;Frank White;Bing Yang Rice xa13 Recessive Resistance to Bacterial Blight Is Defeated by Induction of the Disease Susceptibility Gene Os-11N3 The Plant Cell, 2010, 22(11): 3864-3876 |
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